Cultural Characteristics and Molecular Confirmation of Ustilaginoidea virens the Incitant of False Smut of Rice

Aims: Ustilaginoidea virens, which causes false smut of rice (synonyms: green or pseudo smut), is an emerging grain disease around the world. The main aim study to find out suitable culturable medium, carbon sources and pH for growth pathogen molecular confirmation U. virens isolates.
 Place Duration Study: in vitro studies were conducted Department Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, between December 2020 April 2021.
 Methodology: To pattern U.virens, five different mediums used. Standardization was done using a PSA medium amended with levels. DNA extraction all 13 isolates CTAB method. phylogenetic tree constructed MEGA 12 software.
 Results: It slow-growing grew well potato sucrose agar mycelial about (54.00 mm) after 30 days found be good source. 5 optimum. PCR reaction carried further validate three sets primer pairs ITS 1/ITS 4, US1-5/US3-3, US 2-5/US 4-3 amplified products 560 bp, 380 bp 260 bp. Accession numbers obtained isolates.